Cell 179, 16361646.e15 (2019). Weisel, F. & Shlomchik, M. Memory B cells of mice and humans. New blog post from our CEO Prashanth: Community is the future of AI, Improving the copy in the close modal and post notices - 2023 edition, Manually define clusters in Seurat and determine marker genes, Trim Seurat object to contain expression info only for selected genes, Seurat VlnPlot presenting expression of multiple genes in a single cluster. 5c). Activation dynamics and immunoglobulin evolution of pre-existing and newly generated human memory B cell responses to influenza hemagglutinin. We observed a strong increase in the frequency of S+ and RBD+ Bm cells in SARS-CoV-2-infected individuals at months 6 (median 0.14% and 0.033%, respectively) and 12 post-infection (median 0.068% and 0.02%) compared with acute infection (median 0.016% and 0.0023%) (Fig. ## [15] SeuratObject_4.1.3 Seurat_4.3.0 low.threshold = -Inf, a, Cohort overview of SARS-CoV-2 Infection Cohort. g, Percentages (mean SD) of FcRL4+ Bm cells in paired blood (n=15) and tonsil (n=16) and S+ Bm cells in tonsil samples, separated by SARS-CoV-2-vaccinated (n=8) and recovered patients (n=8). In b, frequencies were compared using a two-tailed Wilcoxon matched-pairs signed rank test. e, Heat map shows enrichment scores of selected gene sets that are significantly different between CD27lo/hiCD21+ resting and CD21CD27FcRL5+ S+ Bm cell subsets in a pseudobulk analysis (n=5 individuals). Med. Policy. Site design / logo 2023 Stack Exchange Inc; user contributions licensed under CC BY-SA. PubMedGoogle Scholar. Can I general this code to draw a regular polyhedron? We used a two-tailed Wilcoxon matched-pairs signed-rank test in b, d and g, and two-sided Wilcoxon test in e. The HolmBonferroni method was used for P value adjustment of multiple comparisons. Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article. Kim, W. et al. Browse other questions tagged, Where developers & technologists share private knowledge with coworkers, Reach developers & technologists worldwide. Colors indicate Bm cell subsets. What were the most popular text editors for MS-DOS in the 1980s? Alice. With Seurat, you can easily switch between different assays at the single cell level (such as ADT counts from CITE-seq, or integrated/batch-corrected data).
